Hi all, I'm not sure how to correctly analyse the following data with glm, and hope for some advice from this list, ideally showing how to specify the model in R and perform the tests, and also for suggestions of literature. The data structure is like this: - 20 plant populations were investigated (random factor pop), which belong to different habitat types (factor ht) - Within each plant population, individuals were grouped into 3 size classes (factor sz) - For each individual, some count data were recorded The independent variables I'd like to analyse are either poission of binomially distributed. For gaussian data, I would use the following model: ht + pop %in% ht + sz + sz:ht + sz : pop %in %ht ht would basically be tested against pop (because the population is the unit of replication for ht), and sz against sz:pop:ht. (the hypotheses to test are that ht has an effect, and whether the effect of sz on individuals of a population depends on ht) However, I do not know how to translate this to the deviance analysis case. For example, when I fit the whole model, and then drop ht to test for the effect of ht, the effect of ht shows up in pop (I understand why, but don't know how to do this otherwise). If I compare the null model to the model including ht only, do I then commit a pseudoreplication? Thanks for your help Pascal