[prev in list] [next in list] [prev in thread] [next in thread] 

List:       bioconductor
Subject:    [BioC] eBayes
From:       "E Motakis, Mathematics" <E.Motakis () bristol ! ac ! uk>
Date:       2004-03-29 15:36:13
Message-ID: 10793281.1080578173 () math-pc167 ! maths ! bris ! ac ! uk
[Download RAW message or body]

Dear all,

In order to perform eBayes command in R, I should log-transfrom the raw 
data so that to be normalized. However, I am not sure if I should also 
standardize them in order to find significant expression with eb$t values. 
Is that the case?

Standardization is the correct procedure or it is enough just to subtract 
log(intensity)- row logmean (without dividing by std dev)?

The SAM procedure requires the same steps?

Thanks in advance,
Makis Motakis


----------------------
E Motakis, Mathematics
E.Motakis@bristol.ac.uk


[prev in list] [next in list] [prev in thread] [next in thread]